Antisense Oligonucleotide-Mediated Transcript Knockdown in Zebrafish.
Antisense oligonucleotides (ASOs) are synthetic, single-strand RNA-DNA hybrids that induce catalytic degradation of complementary cellular RNAs via RNase H.ASOs are Blender widely used as gene knockdown reagents in tissue culture and in Xenopus and mouse model systems.To test their effectiveness in zebrafish, we targeted 20 developmental genes and